I have played guitar for 10 years, but recently had to take over a 1 1/2 year break due to an injury sustained from over-playing. At the time of the injury, it felt like my…
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Best way to inhibit a neurone population?
It is very important to be able to inhibit a neuronal population in order to understand its function. However, I have found it more difficult to achieve than activating neurones. Inhibitory DREADDs (hM4Di) are notoriously…
Performing Real-Time Place Preference
After discussions with a former colleague, I want to try and establish the best way to do real-time place preference (RTPP). My impression was to do this technique as a non-conditioned test, ie. you’re not…
Where to buy in vivo opto kit from?
I bought Plexbright rotary mounted LED’s from Plexon, and have used a collaborator’s Prizmatix LED. Both were blue LED’s and worked fine to stimulate ChR2 in vivo. I’m interested to know what optogenetics equipment anyone…